potfaerie
New Member
- Joined
- Jun 27, 2022
- Messages
- 6
Hello, I am a high functioning autist botanist with an obscene IQ, I just happen to love Mary Jane.
I know what I am talking about, and am not good at convincing people all because they want to argue. If you enjoy the scientific method, growing unheard of amounts of cannabis, of a quality the hippies once worshipped, follow me, read, and feel free to be inquisitive. I will not stand for unsubstantiated urban myths on any of my posts and will gladly block/report/ignore/flame anyone that wants to act up.
I don’t do weird stuff to see if it works (besides when I grew in arborists chip to prove I could). I grow these the way I grow the rest of my plants. Mindful of what the plant wants and need, use sound peer reviewed science to make informed decisions, and use interpretive polyclade logic to proceed into the unknown. Currently I am testing different formulations of IBA for rapid root initiation using a quick dip solution made from pure laboratory grade IBA, and pH adjusted so that the tissue penetrating neutral free-acid is the species most common in solution. This process was informed by my journey to create effective hypochlorous acid for my aerocloner. The history of chlorine and its disinfecting forms is fascinating in and of itself.
Anyway, I started with a tried and true 500ppm IBA solution, diluted from my stock solution of 60% ethanol, 40% water, mixed with approximately 1mL of dry powder. Measuring a dry powder by volume is imprecise, but with manufacturer specifications, molar weight, specific scoop filling process and leveling, it is more than close enough to a 1% solution to be useful, without a scale to measure by mass.
The 500ppm solution is showing to be very efficient at loading the competent cells with IBA so it is available for enzyme conversion to IAA when the plant needs it. Next step will be raising the solution to 1,000ppm.
As a few particular studies have shown that 3000 PPM is effective, and up to 8000 is detrimental, but I have not been able to find any studies that address the pH mediated speciation of IBA into the neutral form that is capable of crossing lipid barriers of cell walls, most effectively. Therefore, I am slowly raising the PPM rather than jumping immediately to 3000, as there is a very good chance that maintaining the low pH speciation of a neutral form, makes more of the IBA immediately available to the cutting, rather than extended soaks, powders, or gels.
What I have found most interesting about the process, is that a quick dip solution, of 5 to 15 seconds, allows for a rapid uptake into the competence cells, but soaking longer than that is intensely detrimental to cutting health and strike rate.
I will rant on later. For now, here are some pics too.
I know what I am talking about, and am not good at convincing people all because they want to argue. If you enjoy the scientific method, growing unheard of amounts of cannabis, of a quality the hippies once worshipped, follow me, read, and feel free to be inquisitive. I will not stand for unsubstantiated urban myths on any of my posts and will gladly block/report/ignore/flame anyone that wants to act up.
I don’t do weird stuff to see if it works (besides when I grew in arborists chip to prove I could). I grow these the way I grow the rest of my plants. Mindful of what the plant wants and need, use sound peer reviewed science to make informed decisions, and use interpretive polyclade logic to proceed into the unknown. Currently I am testing different formulations of IBA for rapid root initiation using a quick dip solution made from pure laboratory grade IBA, and pH adjusted so that the tissue penetrating neutral free-acid is the species most common in solution. This process was informed by my journey to create effective hypochlorous acid for my aerocloner. The history of chlorine and its disinfecting forms is fascinating in and of itself.
Anyway, I started with a tried and true 500ppm IBA solution, diluted from my stock solution of 60% ethanol, 40% water, mixed with approximately 1mL of dry powder. Measuring a dry powder by volume is imprecise, but with manufacturer specifications, molar weight, specific scoop filling process and leveling, it is more than close enough to a 1% solution to be useful, without a scale to measure by mass.
The 500ppm solution is showing to be very efficient at loading the competent cells with IBA so it is available for enzyme conversion to IAA when the plant needs it. Next step will be raising the solution to 1,000ppm.
As a few particular studies have shown that 3000 PPM is effective, and up to 8000 is detrimental, but I have not been able to find any studies that address the pH mediated speciation of IBA into the neutral form that is capable of crossing lipid barriers of cell walls, most effectively. Therefore, I am slowly raising the PPM rather than jumping immediately to 3000, as there is a very good chance that maintaining the low pH speciation of a neutral form, makes more of the IBA immediately available to the cutting, rather than extended soaks, powders, or gels.
What I have found most interesting about the process, is that a quick dip solution, of 5 to 15 seconds, allows for a rapid uptake into the competence cells, but soaking longer than that is intensely detrimental to cutting health and strike rate.
I will rant on later. For now, here are some pics too.
